Vortrag von Ricky Milne am 14.08. 2018

Lr67: A rare hexose transporter variant that confers multi-pathogen resistance… But how?

14.08.2018 at 4.30 pm; HS 6D bldg. 26.11

Speaker bio:

Ricky is a postdoctoral researcher from Evans Lagudah’s team in the Cereal Rust Resistance Group at CSIRO, Canberra, Australia. His research focuses on the mechanism behind the wheat Lr67 hexose transporter variant that confers resistance to multiple pathogens, and testing the function of this variant in other crops. Previously, he investigated transport pathways and functionally characterised sweet sorghum sucrose transporters at The University of Newcastle, Australia under Chris Grof and John Patrick.


Fungal pathogens pose a threat to global wheat production, hence pathogen resistance genes can be deployed to combat disease. Numerous pathogen resistance genes have been identified in wheat, the majority of which are specific to certain pathogen species or even single races of pathogens. Genes which confer resistance to multiple pathogens are rare and poorly understood. An example of a multi-pathogen resistance gene is the hexose transporter Lr67, which confers partial resistance to multiple biotrophic pathogens in wheat – stem rust, stripe rust, leaf rust and powdery mildew. Lr67 belongs to the STP13 clade of monosaccharide transporters and two variants exist, susceptible and resistant, which differ by two amino acids located in transmembrane segments four and ten (G144R, V387L). The susceptible transporter (Lr67sus) was characterised in yeast and has a high affinity to glucose and an acidic pH optimum, similar to other STP13s. However the resistant transporter (Lr67res) was found to be incapable of transporting hexoses and other common sugars when expressed in yeast. To better understand the function of Lr67res, we produced stable transgenic Lr67res barley lines. The Lr67res barley lines exhibited resistance to leaf rust and powdery mildew respectively. The barley plants expressing Lr67res exhibited early senescence and higher pathogen responsive (PR) gene expression. These results confirm that the pathway by which Lr67res functions is conserved between wheat and barley. 

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